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Introduction To ELISA Activity

The ELISA is a fundamental tool of clinical immunology and is used as an initial screen for HIV detection. Based on the principle of antibody-antibody interaction, this test allows for easy visualization of results and can be completed without the additional concern of radioactive materials use.

ELISA activities

An HIV ELISA sometimes called the HIV Enzyme Immunoassay (EIA), is the first and most basic test to determine whether a person is positive for a particular pathogen, such as HIV. To know more about ELISA, you can also browse to check MPO elisa kits.

The test was carried out on a plastic plate measuring 8 x 12 cm containing a matrix measuring 8 x 12 with 96 wells, each with a height of approximately 1 cm and a diameter of 0.7 cm.

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ELISA method

Partially purified and inactivated HIV antigen, coated on ELISA plates Patient serum containing antibodies. If the patient is HIV+, this serum will contain antibodies against HIV and these antibodies will bind to the HIV antigen on the plate.

Enzyme-linked anti-human immunoglobulin. It is the second antibody and binds to human antibodies. A chromogen, or substrate, that changes color when cleaved by an enzyme attached to a second antibody.

False-positive result

It is very likely that a person who is not infected with HIV has antibodies that can give a positive result on an HIV ELISA. This is known as a false positive. One reason is that people (especially women with multiple pregnancies) may have antibodies to the human leukocyte antigen (HLA) present in the host cells used to reproduce HIV.

Because HIV forms pimples from the surface of the host cell, it builds some of the host cell's HLA into its shell. False-negative results can occur during the time window between infection and the antibody response to the virus (seroconversion).